Human PBMNCs (3 × 10 5 /well of 96-well plate (Sarstedt)) (n = 6) were stimulated with phytohemagglutinin (PHA) (32 µg/ml) alone or PHA with different concentrations of CNFs (31.25 µg/ml–1 mg/ml) for 72 h in an incubator with 5 % CO 2. The cultures were pulsed with [3 H]-thymidine for the last 18 h (1 μCi/well, Amersham). At the …

The ultra-fine grinder is a vertical axis reflecting type grinder that can simultaneously complete two processing steps of fine grinding and fine separation. It consists of the …

Cellulose sludge was first suspended in distilled water and dispersed in water to a concentration of 3 % by using a mechanical blender (Silverson L4RT, England) at 3,000 rpm for 10 min. Then, the suspension was passed through an ultra-fine grinder (MKCA 6–3, Masuko, Japan) at 1,440 rpm until a gel was formed. The processing time was 30 min.

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CNFs were isolated from linter pulp using ultrafine grinder, MKCA 6-3, from Masuko (Japan). The rotor speed was 1500 r min 1 . The suspension was passed …

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Subsequently, unmodified nanofibrils, herein termed CNF-0, were obtained by grinding the suspension with an ultrafine grinder (MKCA 6-3, Masuko Sangyo, Kawaguchi, Japan). ... was 18.6 ± 2.3 mmol kg −1, which equals an …

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The swollen fibers were dispersed using a blender for 10 min to get a homogeneous fiber suspension and then diluted with water to a 1 wt% suspension. The cellulose suspensions were then passed for 15 min through an ultrafine grinder (Cerendiptor MKCA 6–3, Masuko, Japan). The distance between the discs was reduced …

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Cellulose sludge was first suspended in distilled water and dispersed in water to a concentration of 3 % by using a mechanical blender (Silverson L4RT, England) at 3,000 rpm for 10 min. Then, the suspension was passed through an ultra-fine grinder (MKCA 6–3, Masuko, Japan) at 1,440 rpm until a gel was formed.

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MKCA 6–3 from Masuko (Japan) at 1440 rpm in contact mode to obtain nanofibres.Oct 01 2014· Thus a suspension was made of 3 wt.% fibres dispersed in water using a mechanical blender (Silverson L4RT JAPAN 332-0012 TEL：81-48 …design of ultrafine grinders.

suspension was grinded using a ultra-fine grinder, MKCA 6-3 from Masuko (Japan) at 3500 rpm in contact mode for approx. 15 min. to obtain cellulose nanofibers. Figure 1 shows the AFM images of the isolated nanofibers, indicating that the fibrillation process was successful and the pulp was isolated to an entangled network of nanofibers.