Single-cell proteomics is a challenging goal and an area of rapid methods development. This Focus issue highlights the many paths toward high-throughput, high-sensitivity measurements. High ...

Nitrogen (N) and phosphorus (P) are two of the key elements for plants and play crucial roles in plant function and ecosystem processes (Sterner and Elser 2002).As the major limiting elements of plant growth, N and P are fundamental components of enzymes, genetic material and cell structure, and are tightly linked to many biochemical processes, …

Microbes Involved in N Fixation. Biological N fixation is the largest natural source of new N to most terrestrial ecosystems (Galloway et al., 2004).In this process symbiotic and free-living diazotrophic microorganisms reduce atmospheric dinitrogen gas (N 2) to reactive and biologically available forms.The nifH gene, encoding the reductase subunit of …

Nitrogen fixation rates were determined using an acetylene reduction assay . During the harvesting of the N 2-fixing plants, fresh nodules were excised from N 2-fixing plant roots and incubated with a conical flask (125 ml; with 10% acetylene atmosphere) for 30 min in situ. A 30 ml gas samples were extracted from the sealed conical flasks using ...

This heterogeneity in turn leads to substantial changes in rates of N 2 fixation and the subsequent addition of newly fixed N to the ocean. As such, spatial (even within relatively localised boundaries) and seasonal shifts in diazotroph diversity and activity must be considered in future regional and global marine N-cycle budgets and modelling ...

A classic nitrogen reduction technology in industry is the Haber–Bosch process, where high temperatures (400–500 °C) and pressures (200–300 atm) are …

Next, we established the connection between NH 4 + limitation and phenotypic heterogeneity in N 2 fixation. We used NanoSIMS to determine the N 2 fixation rates of single cells in populations ...

Nitrogen Fixation as NOx Using Air Plasmas Coupled with Heterogeneous Catalysis Xuekai Pei1* and David B. Graves2 1. Wuhan University, Wuhan, China 2. PPPL, Princeton University and UC Berkeley, USA ... Reduce energy cost by coupling with heterogeneous catalysis 27/36 Packed DBD plasma reactor Patil, B. S., et al. (2016). …

Observed heterogeneity in 15 N uptake by two different unicellular diazotrophs. a, d Diel change in N 2 fixation ( 15 N: 14 N), b, e diel change in percentage of 15 N-enriched cells, c, f diel ...

Aims Increases in anthropogenic nitrogen (N) deposition are expected to lead to an overall higher mean and lower variance of N in the soil, which may disproportionately affect leguminous plants that acquire N both symbiotically via rhizobia and abiotically from soil. Here, we investigate the effects of changing N-heterogeneity at a …

Mechanisms and heterogeneity of in situ mineral processing by the marine nitrogen fixer Trichodesmium revealed by single-colony metaproteomics | ISME …

Biological nitrogen fixation, the conversion of atmospheric N 2 to NH 3, plays an important role in the global nitrogen cycle and in world agriculture [1]. Nitrogen fixation is mainly catalyzed by the Mo-nitrogenase. The ability to fix nitrogen is widely, but sporadically distributed among Archaea and Bacteria which

Nitrogen fixation, the conversion of di-nitrogen (N 2) gas to reactive nitrogen (N) by specialized microbes, plays an essential role in Earth's nitrogen cycle. …

Microbes Involved in N Fixation. Biological N fixation is the largest natural source of new N to most terrestrial ecosystems (Galloway et al., 2004).In …

These composites have been experimentally confirmed as an efficient photocatalytic for nitrogen fixation and transformed from homogeneous SiW 12 to heterogeneous catalyst. The 100-MIL-101(Cr)-I and 100-MIL-101(Cr)-S among them, achieved a high NH 3 yield of 33.93 μmol·h −1 ·g -1 and 75.56 μmol·h −1 ·g -1 , …

The presence of invasive species reduces the growth and performance of native species; however, the linear or non-linear relationships between invasive abundance and native population declines are less often studied. We examine how the amount and spatial distribution of experimental N deposition influences the relationship between non …

A catalyst can increase the rate of a reaction ________. A) by changing the value of the frequency factor (A) B) by increasing the overall activation energy (Ea) of the reaction. C) by lowering the activation energy of the reverse reaction. D) by providing an alternative pathway with a lower activation energy.

Australia's tropical waters represent predicted 'hotspots' for nitrogen (N2) fixation based on empirical and modelled data. However, the identity, activity and ecology of diazotrophs within this ...

In this study, nitrogen fixation rates and diazotroph diversities in sediments with heterogeneous ecological status in one eutrophic lake were …

Biological nitrogen fixation (BNF) in sediments is an important source of bioavailable nitrogen in aquatic systems. However, the effect of habitat change caused by eutrophication on nitrogen fixation within sediments is still unclear. ... This study revealed that habitat heterogeneity in eutrophic lakes shaped different succession of BNF in ...

@article{osti_966122, title = {Influence of heterogeneous ammonium availability on bacterial community structure and the expression of nitrogen fixation and ammonium transporter genes during in situ bioremediation of uranium-contaminated groundwater}, author = {Mouser, P J and N'Guessan, A L and Elifantz, H and Holmes, D …

Great enthusiasm in single-atom catalysts (SACs) for the nitrogen reduction reaction (NRR) has been aroused by the discovery of metal–Nx as a promising catalytic center. However, the poor activity and low selectivity of available SACs are far away from the industrial requirement. Through the first-principles high-throughput screening, we find …

Request PDF | Influence of Heterogeneous Ammonium Availability on Bacterial Community Structure and the Expression of Nitrogen Fixation and Ammonium Transporter Genes during in Situ Bioremediation ...

There are two potential answers to this second question, neither of which exclude the other: ( i) there are phylogenetic constraints to the evolution of late …

Heterogeneous nitrogen fixation rates confer energetic advantage and expanded ecological niche of unicellular diazotroph populations Data availability. The data used to generate the graphs presented in the main figures can be found as …

efficiency for nitrogen fixation by adding heterogeneous catalysts. It appears that fluidizing catalyst particles in the gas flow is viable and worthy of further investigation s. The lowest energy cost that we measured across the different types of discharges or/and coupled with heterogeneous catalysis is about 210 GJ/tN (2.9 MJ/mol).

This review summarizes the recent research on nitrogen fixation, focusing on the active sites for N 2 on the catalyst surface, classifying common active sites, explaining the main role and additional …

Abstract. Spatiotemporal heterogeneity of soil available nitrogen (AN) (sum of NO 3− –N and NH 4+ –N) is the essential basis for soil management and highly correlates to crop yield. Both geostatistical and traditional analyses were used to describe the spatiotemporal distribution of AN in the 0–20-cm soil depth on typical Mollisol ...

Communications Biology - Author Correction: Heterogeneous nitrogen fixation rates confer energetic advantage and expanded ecological niche of unicellular diazotroph populations

The overall goal of this dissertation was to evaluate how spatial heterogeneity of environmental variables 1) drive hot spots of N2 fixation, denitrification rates and gene abundances in streams ...